CURIOX APPLICATIONS
MASS CYTOMETRY
Improve your mass cytometry data by reducing background and enhancing cell retention.
- Home
- Applications _ Mass Cytometry
Overview
Change the way you process cells to get reliably consistent data.
Mass cytometry is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than labeled with fluorescent antibodies. Since detection is by time-of-flight mass spectrometry, there is no spectral overlap of signals, which allows for higher numbers of target proteins to be analyzed.
Mass cytometry allows deep studies of both surface markers and intracellular cytokines. It is a well-documented method with many publications. Most often you will find a mass cytometry in an institution’s core facility, so preparing your samples in your own lab is key to getting good results.
“The one thing which I didn't expect it here is that you reduce usage of antibody with roughly 50% and we've preserved signal and reproducibility.”
Traditional centrifugation
VS
Laminar flow technology
Mass cytometry protocols can be quite harsh on the cells and Laminar Wash technology treats them gently as compared to centrifugation, which can result in more singlets.
Benefits
Benefits of laminar wash for mass Cytometry
Mass cytometry experiments are expensive and time consuming. Make sure all of your samples produce good data with Laminar Wash technology.
- Reduce debris, background, artifacts and clogging
- Enhance cell retention
- Improve resolution
- Lower the CVs of data
Featured Resource
BENEFITS OF USING THE CURIOX LAMINAR WASH™ SYSTEM IN MASS CYTOMETRY EXPERIMENTS
Scientific Data
Data using the laminar wash system
Generate More Intact Singlets Compared to Centrifugation
A 25 isotope-labelled panel was used on PBMC samples starting with one million cells. Samples were then prepared using either Laminar Wash technology or centrifugation.
The frequency of singlets is higher in the Laminar Wash prepared samples. The number of intact cells was also significantly greater with the Laminar Wash treated samples.
Increased Reproducibility Compared to Centrifugation
RELATED PRODUCTS AND ACCESSORIES
Curiox Laminar Wash
LAMINAR WASH™ HT2000 SYSTEM
Achieve higher throughputs and cleaner data with our 96-well laminar washing system.
Curiox Laminar Wash Auto
LAMINAR WASH™ AUTO 1000 SYSTEM
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
