Mass cytometry is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than labeled with fluorescent antibodies. Since detection is by time-of-flight mass spectrometry, there is no spectral overlap of signals, which allows for higher numbers of target proteins to be analyzed.
Mass cytometry allows deep studies of both surface markers and intracellular cytokines. It is a well-documented method with many publications. Most often you will find a mass cytometry in an institution’s core facility, so preparing your samples in your own lab is key to getting good results.
Mass cytometry protocols can be quite harsh on the cells and Laminar Wash technology treats them gently as compared to centrifugation, which can result in more singlets.
Mass cytometry experiments are expensive and time consuming. Make sure all of your samples produce good data with Laminar Wash technology.
A 25 isotope-labelled panel was used on PBMC samples starting with one million cells. Samples were then prepared using either Laminar Wash technology or centrifugation.
The frequency of singlets is higher in the Laminar Wash prepared samples. The number of intact cells was also significantly greater with the Laminar Wash treated samples.
Achieve higher throughputs and cleaner data with our 96-well laminar washing system.
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
Avoid the centrifuge and improve data quality and biosafety with the Laminar Wash MINI System.