The World Health Organization Laboratory guidance for research on the novel coronavirus SARS-CoV2 states, “all technical procedures should be performed in a way that minimizes the generation of aerosols and droplets.”
|Table 1. Risk prioritization of selected routine laboratory tasks|
|Task or activity||Exposure risk|
|Potential hazard||Likelihood||Consequence||Risk rating|
|Subculturing blood culture bottle||Needle stick — percutaneous inoculation||Likely||Infection; medical treatment||High|
|Aerosols — inhalation||Moderate||Infection; medical treatment||Medium|
|Splash — direct contact with mucous membranes||Moderate||Infection; medical treatment||High|
|Centrifugation||Aerosols — inhalation||Likely||Infection; medical treatment||High|
|Performing Gram stain||Aerosols from flaming slides||Moderate||Colonization; infection||Moderate|
|Preparing AFB smear only||Aerosols from sputum or slide preparation||Likely||Illness; medical treatment; disease||High|
|Performing catalase testing||Aerosols — mucous membrane exposure||Unlikely||Colonization; infection||Low|
|AFB culture work-up||Aerosols — inhalation||Likely||Illness; medical treatment; disease||High|
|Abbreviation: AFB = acid-fast bacillus.|
Curiox Biosystems introduces a new, safer method for preparing potentially or known infectious blood samples for immune cell profiling without a centrifuge, eliminating aerosolization of potentially infectious samples. The method combines a new configuration of the Laminar Wash HT2000 system packaged with STEMCELL Technologies EasySep RBC depletion reagent to enable blood sample processing within a biosafety cabinet from start to finish.
This natural and gentle cell prep method relying on Laminar Wash technology improves viability, retention, and staining resolution of rare and sensitive immune cell subsets known to play crucial roles in the immune response against COVID-19 infections, like monocytes and neutrophils. Improvements in data quality due to reduced stress are evident even on fragile cells resulting from harsh methods such as red blood cell lysis that relies upon repeated centrifugation steps.
Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.
Laminar Wash technology was compared to centrifugation protocols in a 16-color immune profiling panel using 100μL of whole human blood. One-step washing using Laminar Wash minimized cell perturbations compared to multiple centrifugation steps, increasing retention of both the monocyte and neutrophil populations.
When performing cell dissociation in tumor-bearing tissues, tissue debris and enzymatic-induced fragments are primary factors affecting recovery of tumor infiltrating lymphocytes (TILs). For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs hinders accurate characterization. Here, Laminar Wash technology was compared to conventional methods for preparing an unchallenged mouse sample containing ~1,000,000 tumor infiltrate cells. Laminar Wash improved viable cell count and reduced debris formation. The cleaner sample preparation increased lymphocyte recovery up to 90.6%, compared to 10.5% for the centrifuge.
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle, Discovery Research Services at Charles River Laboratories, Worcester, MA
Achieve higher throughputs and cleaner data with our 96-well laminar washing system.
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
Avoid the centrifuge and improve data quality and biosafety with the Laminar Wash MINI System.