CURIOX PRODUCTS
LAMINAR WASH™ HT2000 SYSTEM
Achieve higher throughputs and cleaner data with our 96-well laminar washing system.
With the Curiox Laminar Wash HT2000 System, you get all the benefits of gentle laminar cell washing in a format compatible with high-throughput and automated workflows.
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- Laminar Wash™ HT2000 System
Laminar Wash System
What the HT2000 delivers
- Better data quality and better cell recovery than centrifugation
- A higher stain index for cleaner resolution of populations
- Faster workflows that can completely wash 96 samples in just 3 minutes
- Better data quality and better cell recovery than centrifugation
- Greater reproducibility from study-to-study and operator-to-operator
- A higher stain index for cleaner resolution of populations
- Greater reproducibility from study-to-study and operator-to-operator
- Less cell clumping and the subsequent clogging of fluidic systems
- Better biosafety through reduced aerosolization and a footprint that fits into most biosafety cabinets
- Less cell clumping and the subsequent clogging of fluidic systems
- Faster workflows that can completely wash 96 samples in just 3 minutes
- Better biosafety through reduced aerosolization and a footprint that fits into most biosafety cabinets
Technology overview
A quicker, simpler path to better data
“With the Curiox Laminar Wash™ we retain more cells with much less data variation between samples than our centrifuge process.”
Traditional workflow
VS
Laminar flow technology
Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the HT2000, you can get complete washing of 96 samples in just 3 minutes.
How our plate and nozzle design wahes your cells
The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.
Scientific Data
Laminar washing delivers better cell retention and cleaner, clearer data
The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
Laminar wash advances with neutrophils and monocytes retention for improved differential blood count
Figure 2. Human red blood cells (RBCs) lysed with (NH4Cl) and washed using HT2000 Laminar Flow technology (top) show better cell retention and tighter cell populations than RBCs lysed and washed using centrifugation on samples in tubes (bottom), whether enumerated by CD45 staining or FSC-SSC scattering.
TECHNICAL SPECIFICATIONS
Voltage Requirements | 100-240V |
Power Consumption | 24V/3.0A |
Dimensions | 310 mm H x 262mm W x 302 mm D |
Weight | 14kg (31lbs) |
ACCESSORIES AND COMPONENTS
Laminar Wash™ 96-well plate (with a regular lid) | 96-DC-CL-05 |
Laminar Wash™ 96-well plate (with a regular lid) *Customised | 96-DC-CL-05 |
HT Buffer Exchanger 5 | DC-BX-01-05 |
HT Buffer Exchanger 10 | DC-BX-01-10 |
