The Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. You get all the benefits of laminar washing in a completely automated system.
Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the AUTO 1000, you can prepare 16 samples in just 6 minutes.
Complement-dependent cytotoxic crossmatch (CDCXM) and flow cytometric crossmatch (FXCM) are both common methods to predict donor-recipient compatibility – the potential success of an organ transplant. FCXM assays are more widely applied across human leukocyte antigen (HLA) labs. FXCM utilizes the use of multiple washing steps, serum treatment and secondary antibody used. Results between the centrifugation-based and Laminar Washing methods within 8 replicates and 3 test sera and 2 controls show average CVs of 12.4% vs 7.9% respectively. With Laminar Wash technology, greater consistency was seen on the Median Channel Fluorescence values gated on B-cells. The ratio of positive crossmatch yielded a higher shift while maintaining a lower MCF value for the negative background. This is attributed by a superior wash and accuracy in matrix serum handling with primary patient cells.