Drug Discovery / Functional Genomics

Pooled high content phenotypic screens of genes and drugs. Compatible with multiple libraries including CRISPR gRNAs, shRNAs, antibodies, and even small molecule compounds.

Pooled High-Content Screening of Genes and Drugs

VisionSort Solution for Drug Discovery

From array to pool

Image-based high-content screening (HCS) is a powerful method for identifying genes compounds that induce physiologically relevant disease-linked cellular phenotypes. However, it is time-consuming expensive due to its reliance on well-to-well imaging of microplates.

ThinkCyte has developed a new method that enables high-throughput image-based HCS in a pooled format using their proprietary machine Vision-based Cell Sorting technology. This novel method is compatible with various types of libraries, including CRISPR gRNAs, shRNAs, antibodies, peptides, small molecule compounds.

* Images acquired from the FlowSight system (Luminex)

Key Advantages of ThinkCyte Technology

Compatible with a wide range of libraries

Small molecule libraries, CRISPR, shRNA, peptide, phage libraries, etc.

High speed sorting

・Pathway-specific libraries: 1–3 h ・Whole-genome libraries: 8–12 h ・Compound libraries (~100,000 compounds): 1–3 d

For precious cells

Only a few hundred cells per gene/compound are required for screening.

Link genotypes to phenotypes

Image data is linked to the NGS data.

Phenotype-driven Target Validation in Drug Discovery

In the phenotypic screening process, identification of disease relevant phenotypes is crucial. Our screening platform is able to detect various types of phenotypes, such as changes in protein localization, aggregation, and cell and organelle morphology, using fluorescence reporters.

Furthermore, our proprietary machine vision-based classification technique can discriminate morphological differences of cells including live and apoptotic cells, differentiated and undifferentiated cells, and healthy and disease relevant cells, without the use of molecular labels. These label-free phenotypes can be also used in our screening system.

Screening Workflows

ThinkCyte screening platform enables image-based high-content screens in a pooled format and can dramatically accelerate the screening process while reducing costs.

It is compatible with a wide range of libraries, including CRISPR gRNAs, shRNAs, antibodies, peptides, and small molecule compounds.

Pooled screening of genes

ThinkCyte technology is applicable for genetic screens using lentivirus libraries, including CRISPR libraries.

Pooled screening of drugs

Combining ThinkCyte proprietary Ghost Cytometry technology with DNA barcoding and droplet techniques, image-based high-content screens can be performed for small molecule libraries in a pooled format as well.

Images by ThinkCyte

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