This family of fluorescent dyes can be used on both spectral and conventional flow cytometers. Designed for ease of use, KIRAVIA Dyes™ do not require a special buffer system or sample preparation protocol.
To further life science research in the regenerative medicine and immunology fields, Sony has released a portfolio of dyes conjugated to antibodies, giving researchers new choices for panel design. The conjugates can be used in a variety of multicolor applications.
The first dye in the family, KIRAVIA Blue 520™ (KB520), is excited by the 488-nm laser, with a maximum signal at 520 nm. Recommended for experiments on flow cytometers equipped with a 488-nm laser, this novel dye is ideal for panels with dim expressors. Its excellent brightness provides better discrimination and resolution. KB520 can replace fluorochromes that use the 530/30 (FITC) detector.
In high parameter experiments KB520 can be used to detect antigens with a variety of abundance levels, but would ideally be saved for an antigen with ubiquitous and/or variable expression levels in the sample. The KB520 conjugated antibodies exhibit higher stability and brightness compared to FITC conjugates.
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